摘要:在利用芸芥自交不亲和系途径开展育种研究的过程中 ，为了避免在人工试配组合时，双亲蕾期杂交结籽率较好，但在自然隔离条件下配制杂交种 时出现双亲花期交配不亲和的现象，本试验对10份芸芥进行了系内株间和系间的完全双列杂 交，同时对自交不亲和基因的表达器官进行了DDRT-PCR扩增研究。结果表明，芸芥1、芸芥3 、芸芥5、芸芥6、芸芥8、芸芥9和芸芥13这7份材料系内株间异交和套袋自交的亲和指数均 小于1，即表现为不亲和性，说明这些材料属于S等位基因纯合的不亲和系；而芸芥2、芸芥4 和芸芥10这三份材料系内株间异交和套袋自交时，呈现出不亲和性不稳定的现象，即一部分 杂交表现为亲和，另一部分杂交表现为不亲和，说明这3份材料的自交不亲和性尚未稳定。D DRT-PCR扩增结果表明，芸芥自交不亲和S基因只在柱头组织中表达，其属于组织特异性表达 。因此，在利用自交不亲和系途径培育一代杂种时，在农艺学性状和自交不亲和性基本稳定 时，有必要进一步测定自交不亲和系S等位基因的纯合性和基因型，这一工作可以有效地指 导育种实践。

关键词:芸芥  自交不亲和系  S等位基因  基因表达  DDRT-PCR

AbstractIn the process of breeding research by ways of sel fincompatible lines in E. Sativa Mill., there is often a phenomenon that t he seed setting percentage from the cross of two parents is high in bud stage, b ut the number of seeds is low in late stage owing to the natural segregation. By using the method of complete diallel crossing, the purity of S alleles in 10 se lfincompatible lines of E. Sativa Mill. was studied, and the expression ti ssue of selfincompatible genes was analyzed with DDRT-PCR technique. The resul ts showed that the selfcompatible index of Yunjie 1, Yunjie 3, Yunjie 5, Yunji e 6, Yunjie 8, Yunjie 9 and Yunjie 13 was below 1 after selfing or outcrossing among these 7 selfincompatible lines, that is to say, their selfincompatibil ity was stable. However, the selfincompatibility of Yunjie 2, Yunjie 4 and Yun jie 10 was not stable. DDRT-PCR analysis showed that the selfincompatible gene s of E. Sativa Mill. didn’t belong to constitutive expression, but to speci fic tissue expression. Therefore, when creating F1 hybrids by ways of selfinco mpatible lines, it is necessary for us to determinate the purity of S alleles an d their genotypes besides agronomic traits and selfincompatible stability.

Key wordsEruca sativa Mill.  self incompatible lines  S al leles  gene expression  DDRT-PCR

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn