摘要:采用盆栽土壤基施0、15、30 kg·hm^-2三个氮 素水平试验，研究了芸豆荚果形成后不同氮素水平下叶片和荚果的碳、氮代谢主要酶活性 以及抗氧化酶系活性的变化。结果表明，芸豆荚果形成后，随着土壤供氮水平的增加，荚果 的磷酸烯醇式丙酮酸羧化酶活性、多酚氧化酶活性、超氧化物歧化酶和过氧化氢酶活性逐渐 增强；叶片的蔗糖合成酶活性、蔗糖磷酸合成酶活性、硝酸还原酶和磷酸烯醇式丙酮酸羧化 酶活性大于同一供氮水平荚果的活性；且荚果的总可溶性糖和可溶性蛋白含量高于叶片；叶 片的磷酸烯醇式丙酮酸羧化酶的活性与荚果的蔗糖磷酸合成酶和硝酸还原酶活性呈显著相关 性。由此可以得出，源器官是芸豆植株的代谢活跃中心，而库器官成为植株的生长中心，且 后者的碳、氮代谢过程主要受前者的调控。

关键词:芸豆  氮素  碳氮代谢  代谢平衡

AbstractThree base nitrogen fertilizer levels, including 0 , 1.5 kg·hm^-2 and 3.0 kg·hm^-2, were adopted in a pot experiment to study the changes of enzyme system of carbonnitrogen metabolism in leaves an d pods of kidney bean (Phaseolus vulgari L.). The results showed that, after the formation of pods, the activity of phosphoenolpyruvate carboxylase (PEP), p olyphenol oxidase (PPO), superoxide dismutase (SOD) and catalase (CAT) in pods g radually increased with the increase of nitrogen. Under the same nitrogen level, the activity of sucrose synthase (SS), sucrose phosphate synthase (SPS), nitrat e reductase (NR) and PEP in leaves was greater than that in pods, while the cont ent of total soluble sugar and protein in pods was higher than that in leaves. T he activity of PEP in leaves was correlated significantly with the activity of S PS and NR in pods. It could be concluded that, in the plants of kidney bean, the source organ was the active center of metabolism, while the sink organ was the growth center, and the carbonnitrogen metabolism process of the latter was mai nly regulated by the former.

Key wordskidney bean  nitrogen  carbon-nitrogen metabolism  met abolic balance

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn