摘要:以马铃薯不同基因型品种脱毒基础苗为试验材料，采用两因素随机区组设计,研究不同基因型品种、不同抗生素浓度对马铃薯脱毒基础苗苗鲜重、根鲜重、苗干重、根干重、叶片数、节数、株高、茎粗、根长和根条数等农艺性状影响，培养14 d时，测定各性状指标。结果表明：3个不同成熟期基因型品种对测定的10个农艺性状指标影响显著(P<0.05)；4种抗生素浓度（0、20、40、60 mg·L^-1）除了茎粗 ，对其它9个农艺性状指标影响显著(P<0.05)，适宜的抗生素浓度范围为0 mg·L^-1和20 mg·L^-1；3个不同成熟期基因型品种、4种不同抗生素浓度处理对马铃薯 单株苗鲜重、根鲜重、苗干重、根干重、节数、株高、茎粗、根条数影响有显著性交互作用 (P<0.05)，叶片数、根长不受基因型品种和抗生素浓度交互作用影响；早熟基因型品种 大西洋、中熟基因型品种克新1和晚熟基因型品种青薯168适宜的抗生素浓度为0、20 mg·L ^-1，各农艺性状表现好。为预防污染发生，降低生产成本，建议继代3-5代时,加入抗 生素20 mg·L^-1。

关键词:马铃薯  品种  抗生素  生长

AbstractThe different varieties of virusfree potato plan tlets were selected as the meterials, and using the method of randomized block d esign of two factor to study the effects of genotypes and different antibiotic concentrations on the fresh weight of shoot and root, the dry weight of shoot an d root, leaf number, node number, plant height, stem diameter, root length and r oot number of individuals after 14 d culture. Our results showed that genotypes had significant effects on the ten agronomic traits (P<0.05). Four kinds of antibiotic concentrations（0、20、40、60 mg·L^-1）had notable interacti on effects on other agronomic characters eccept stem diameter (P<0.05), the suitable antibiotic concentrations were 0 mg·L^-1 and 20 mg·L^-1. Ex cept for leaf number and root length properties index, the genotypes and antibio tic concentrationshad notable interactions on other agronomic characters eccept (P<0.05). The suitable antibiotic concentrations for the earlymaturing va rietie “Atlantic”, mediummaturing varietie “Kexin 1” and latematuring va rietie “Qingshu 168” were 0 mg·L^-1 and 20 mg·L^-1, to prevent the pollution and production reduction, we suggested to add 20 mg·L^-1 antibi otic if subcultured for 3-5 generations.

Key wordspotato  variety  antibiotic concentration  growth

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn