摘要:以己唑醇为配体与醋酸铜配合获得元素组成异化的己唑醇铜配合物。配合物对小麦种子浸种处理，待萌发生长至幼苗阶段将其进行NaCl胁迫处理，通过对幼苗脯氨酸含量以及脯氨酸合成酶基因P5CS、P5CR表达变化的测定，评价己唑醇铜配合物对植物抗盐能力的影响。结果表明：小麦幼苗脯氨酸含量随着盐浓度的增大及胁迫时间的延长而增加，200 mmol·L^-1 NaCl胁迫下，配合物处理后脯氨酸含量最高，达55.44 μg·g^-1，相比己唑醇和醋酸铜处理分别增加13.68%和58.45%；而100 mmol·L^-1 NaCl处理6 h，己唑醇铜配合物处理小麦幼苗的脯氨酸合成增幅最大，P5CS、P5CR表达量明显增加，在一定程度上缓解了小麦幼苗的渗透胁迫伤害。

关键词:己唑醇铜配合物  脯氨酸  半定量PCR  盐胁迫

AbstractThe hexaconazole-Cu complex, synthesized by hexaconazole and copper acetate, was used to soak the seed of wheat to ameliorate the wheat seedlings salinity stress induced by sodium chloride (NaCl). The effect of hexaconazole-Cu complex on the anti-salinity stress of wheat seedlings was evaluated by proline accumulation level and the gene expression level of Δ1-pyrroline-5-carboxylate synthetase ( P5CS) and Δ1-pyrroline-5-carboxylate reductase (PC5R) of wheat seedlings. The results showed that in the presence of 200 mmol·L^-1 NaCl, the proline content of the 0.03 mmol·L^-1 complex treated wheat seedlings was 55.44 μg·g^-1, which was 13.68% higher than that in hexaconazole treatment, and 58.45% higher than that in copper acetate treatment. The gene expression levels of P5CS and P5CR were quantitated by semiquantitative RT-PCR analysis. The results showed that the hexaconazole-Cu complex enhanced the transcript levels of P5CR and P5CS. After 6 h treatment of the complex in the presence of 100 mM NaCl, the P5CS express level reached the maximum, and was significantly higher than in hexaconazole treatment. It was obvious that the hexaconazole-Cu complex enhanced the proline contents, thereby played a protective role in the salinity stress of wheat seedlings.

Key wordshexaconazole-Cu complex  proline  semiquantitative RT-PCR  salt stress

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

投稿时间：2012-08-17修订日期：2012-12-20 改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn