摘要:为探究一氧化氮(NO)和水孔蛋白(AQP)在种子萌发过程中是否存在互作效应，以小麦品种陕合6号（耐旱型）和郑引1号（干旱敏感型）为材料，研究H₂O、HgCl₂（500 μM）、PEG（PEG-8000，-0.35 MPa）、SNP（0.5 mM）、SNP+PEG、SNP+HgCl₂溶液浸种对种子萌发的影响。结果表明：SNP(NO供体)促进了抗旱品种种子的萌发，而抑制了干旱敏感品种种子的萌发；AQP在干旱敏感品种种子萌发中的作用大于抗旱品种；NO能够 解除干旱和水孔蛋白抑制剂HgCl₂对种子萌发的抑制作用；处理对吸胀种子萌发的抑制效应小于直接在处理液中萌发的抑制效应；NO对AQP的活性具有调节作用且NO和AQP的作用主要存在于种子最初吸水萌动的12 h内。

关键词:NO  水孔蛋白  种子萌发  小麦

AbstractIn order to explore the interactions between nitric oxide (NO) and aquaporin (AQP) in seed germination, two wheat varieties including Shaanhe No.6 (SH6, drought-resistant) and Zhengying No.1 (ZY1, drought-susceptible) were adopted to study the effects of H₂O, HgCl₂(500 μM), PEG (PEG-8000, -0.35 MPa), SNP (0.5 mM), SNP+PEG and SNP+HgCl₂ solution soaking on seed germination. The results showed that SNP (NO donor) promoted seed germination of the drought-resistant variety, while inhibited that of the drought-susceptible variety. AQP played a more important role in the drought-susceptible variety than in the drought-tolerance variety. NO could relieve the inhibition of seed germination caused by drought or aquaporin inhibitor (HgCl₂). The inhibition degree of stress to germination of the seeds being imbibed was smaller than that of the seeds being germinated directly. NO could regulate the activity of AQP, and both NO and AQP exerted their role mainly in the initial 12 h of germination.

Key wordsnitric oxide(NO)  aquaporin(AQP)  seed germination  wheat

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

投稿时间：2012-03-22修订日期：2012-03-30 改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn