| 摘要: |
| 研究了Cd2+（10 mg·L-1、30 mg·L-1）胁迫下不同浓度Ca2+（0、80、160、320 mg·L-1）对板蓝根种子萌发、幼苗抗氧化酶系统及蛋白质含量的影响。结果表明：低浓度Ca2+（80、160 mg·L-1）可缓解Cd2+毒害，显著提高板蓝根种子的发芽率、发芽势、发芽指数、活力指数，促进蛋白质含量的增加，提高SOD、POD、CAT活性，且160 mg·L-1 Ca2+缓解效果最好，缓解能力随Cd2+浓度的升高有所下降；高浓度Ca2+（320 mg·L-1）与Cd2+作用,反而抑制了板蓝根种子的萌发，幼苗的POD、SOD、CAT活性及蛋白质含量下降。低浓度Ca2+可以显著提高板蓝根的抗性，对Cd2+毒害起缓解作用，高浓度的Ca2+与Cd2+对板蓝根种子起协同毒害作用。 |
| 关键词: 板蓝根 镉 钙 种子萌发 抗氧化酶系统 |
| DOI： |
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| 基金项目:河西学院青年教师科研基金项目（No.QN2012-13） |
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| The effect of Ca2+ on Radix isatidis seed germination and seedling physiological characteristics under Cd2+ stress |
| MENG Hong-mei, ZHANG Fen-qin, HAN Duo-hong, LUO Yin-mao |
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| Abstract: |
This study aims to investigate the effect of different Ca2+ levels (0, 80, 160 mg·L-1 and 320 mg·L-1) on seed germination, activities of SOD, POD, and CAT, and protein content of Radix Isatisdis seedlings under different Cd2+ stress le
vels (10 mg·L-1 and 30 mg·L-1). The results show that low concentration of Ca2+, especially the concentration of 160 mg·L-1, may mitigate the deleterious effect of Cd2+ by increasing germination rate and vigour index, and enhancing activities of SOD, POD, and CAT, and increasing protein content. The influence of Ca2+ on the Cd2+ toxicity was not obvious at high Cd2+ concentration because of the inhibition of the above indexes. These results suggest that the low concentration of Ca2+ could be favorable for coping with Cd2+ toxicity, whereas high concentration of Ca2+ shows synergistic effect with Cd2+ for Radix Isatisdis. |
| Key words: Radix isatidis Cd2+ Ca2+ seed germination antioxidant enzymes |
