| 摘要: |
| 以‘津优4号’黄瓜为试验材料，研究施用不同作物秸秆对衰老过程中黄瓜根系活力和叶片氮代谢关键酶、叶片氮素含量及可溶性蛋白含量等影响。结果表明，与对照相比，作物秸秆还田不仅显著提高了黄瓜的根系活力、叶绿素含量和叶片氮代谢关键酶硝酸还原酶、谷氨酰胺合成酶、谷氨酸合成酶的活性，降低了谷氨酸脱氢酶活性，而且还降低了叶片铵态氮的含量(15.6 μg·g-1)，提高了叶片硝态氮、可溶性蛋白及游离氨基酸的含量，有效地保持较高的氮代谢水平，延缓了黄瓜的衰老。其中施用玉米秸秆效果最好，黄瓜的根系活力、硝酸还原酶活性、可溶性蛋白含量分别比对照提高了23.4%、33.3%、18.7%，其次为花生秸秆，硝酸还原酶活性比对照提高了10.8%，施用稻壳效果不明显。 |
| 关键词: 作物秸秆 黄瓜 衰老 根系活力 氮代谢 |
| DOI： |
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| 基金项目:安徽省自然科学基金项目(1208085QC55)；安徽省科技学院引进人才基金项目(ZRC2009250) |
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| Effects of crop straw on root activity and leaf nitrogen metabolism of cucumber during senescence |
| GAO Qing-hai, WANG Ya-kun, LU Xiao-min, MIAO Yong-mei |
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| Abstract: |
| The study aims to investigate the effects of different crop straw on key enzymes of nitrogen metabolism of root system and leaves, nitrogen content and soluble protein content in leaves of Jinyousihao during senescence. The results showed that, compared with the control, crop straw significantly increased the root activity, chlorophyll content, nitrate reductase, glutamine synthetase and glutamate synthase activity, while it reduced the glutamate dehydrogenase activity and the content of ammonium nitrogen in leaves (15.6 μg·g-1). The nitrate content, soluble protein and free amino acid content increased, which effectively maintained a higher level of nitrogen metabolism and delayed the senescence of cucumber. The effect of corn straw returning was the best, for the root activity, nitrate reductase activity and soluble protein content the this treatment were increased by 23.4%, 33.3% and 18.7%, respectively, as compared with the control. Peanut straw showed better effect, with nitrate reductase activity increased by 10.8%. By contrast, rice straw returning showed no significant effect. |
| Key words: straw cucumber senescence root activity nitrogen metabolism |
