摘要:对卫星搭载的苜蓿叶片诱导产生愈伤组织进行干旱胁迫，探讨苜蓿愈伤组织对PEG胁迫的响应和抗氧化酶活性等生理指标的变化。结果表明：随着PEG浓度的升高，苜蓿愈伤组织的致死率逐渐增加，25%、30%PEG浓度分别为愈伤组织半致死浓度和致死浓度。卫星搭载处理提高了苜蓿愈伤组织可溶性糖、可溶性蛋白和脯氨酸含量，增加了POD和SOD活性，降低了丙二醛含量，且与对照差异均显著。综合各项生理生化指标卫星搭载可以提高苜蓿抗旱性，可获得高抗旱性苜蓿突变体。

关键词:苜蓿  愈伤组织  卫星搭载  PEG胁迫  生理特性

AbstractIn order to improve the alfalfa's resistance to drought, the drought stress was simulated on leaf callus of alfalfa that was carried by satellite, and the response to PEG stress and 6 physiological and biochemical indexes were measured. The results showed that the rate of fatality increased gradually on alfalfa callus, with half lethal concentration and lethal concentration of PEG being 25% and 30%, as PEG concentration increased. In comparison with CK, satellite carrying significantly improved the contents of soluble sugar, soluble protein, and increased the POD and SOD activity, reduced the relative conductivity and malondialdehyde content. Satellite carrying increased drought resistance of the alfalfa callus, which could be useful for obtaining alfalfa mutants with high drought resistance.

Key wordsalfalfa  callus  satellite carrying  PEG stress  physiological characteristic

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn