摘要:本研究目的在于分析种子引发处理后黄芪发芽特性及幼苗抗旱性，探索提高黄芪种子萌发率的方法，为人工栽培黄芪提供技术支撑。采用20% PEG-6000、0.25% KNO₃、100 mg·L^-1 GA3、12.5 mg·L^-16-BA及H₂O（对照）5种化学物质引发处理黄芪种子，进行萌发试验及干旱胁迫，测定种子萌发特性及有关抗旱生理指标。结果表明：各处理发芽势、发芽率的一致顺序为12.5 mg·L^-16-BA ＞100 mg·L^-1 GA3＞0.25% KNO₃＞20% PEG-6000＞H₂O。幼苗丙二醛(MDA)含量、可溶性蛋白含量、脯氨酸含量、叶绿素含量、超氧化物歧化酶活性（SOD）、过氧化物酶活性（POD）和过氧化氢酶活性（CAT）与CK之间存在显著差异。比较各处理黄芪幼苗的隶属函数平均值可看出，不同引发处理黄芪种子，幼苗的抗旱性顺序为H₂O＞20% PEG-6000＞0.25% KNO₃≈12.5 mg·L^-1 6-BA＞CK＞100 mg·L^-1 GA3。结论：采用20% PEG-6000或0.25% KNO₃作为引发剂处理黄芪种子，可有效促进黄芪种子萌发和提高幼苗抗旱性。

关键词:黄芪  引发处理  萌发  抗旱性

AbstractObjective: Physiological effects of seed priming on drought resistance of Astragalus membranaceus (Fisch.) Bge. seedlings were studied in this paper. Method: After Astragalus membranaceus (Fisch.) Bge.seeds were subject to different priming with H₂O, 20% PEG-6000,0.25% KNO₃,100 mg·L^-1 GA3 and 12.5 mg·L^-1 6-BA respectively for germination experiment, seedlings were under 20% PEG-6000 simulated drought stress to measure d roughtrelated physiological indicators. Results: The sequence of Germination energy and germination percentage was identical: 12.5 mg·L^-1 6-BA＞100 mg·L^-1 GA3＞0.25% KNO₃＞20% PEG-6000＞H₂O＞CK; and the sequence of Simplified vigor index was 0.25% KNO₃＞100 mg·L^-1 GA3≈20% PEG-6000＞H2O＞12.5 mg·L^-1 6-BA＞CK. Malondialdehyde (MDA) content, soluble protein content, proline content, chlorophyll content, superoxide dismutase (SOD), peroxidase(POD) and catalase activity (CAT) of seedling have a significant difference between CK.According to comparing the Average value of subordinate function, the rank of drought resistance was H₂O＞20% PEG-6000＞0.25% KNO3≈12.5 mg·L^-1 6-BA＞CK＞100 mg·L^-1 GA3. Conclusion: 20% PEG-6000, or 0.25% KNO₂ can effectively promote seed germination and improve seedling droughtresistance.

Key wordsAstragalus membranaceus (Fisch.)Bge.seed  priming treatments  permination  drought resistance

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn