摘要:采用钵盘育苗试验，研究了哈茨木霉菌在调节水稻苗床土壤微生物群落及土壤酶活性中的作用。研究结果表明：哈茨木霉菌能有效调节水稻幼苗根际土壤微生物群落组成；哈茨木霉菌对细菌、真菌和放线菌数量的影响程度明显不同，播种28天后，哈茨木霉菌接种处理的水稻幼苗根际土壤细菌和放线菌数量较对照分别增加50.70%和48.56%，而真菌数量较对照减少16.15%，并且哈茨木霉菌数量较刚播种时增加了138.46%；哈茨木霉菌也能显著提高土壤脲酶、磷酸酶和蔗糖酶的活性，分别较对照提高8.55%、18.31%和49.61%。研究表明，哈茨木霉菌具有改善土壤微生态环境的作用。

关键词:哈茨木霉菌  水稻  土壤微生物  土壤酶活性

AbstractThe role of Trichoderma harzianum in regulating soil enzymatic activities and soil microbial community were studied by using a pot seeding experiment. The results showed that T. harzianum could effectively regulated soil microbial community in rhizosphere soil during rice seedling stage, however, it produced different effects on bacteria, fungi and actinomycete. In the 28th day after sowing, the amount of bacteria and actinomycete under inoculation treatment was increased by 50.70% and 48.56%, respectively, but the amount of fungi was reduced by 16.15%, compared with those under control; moreover, the amount of T. harzianum under inoculation treatment was 138.46% more than that in the sowing day. T. harzianum could also improve the activities of urease, ph osphatase and invertase, which were increased by 8.55%, 18.31% and 49.61%, respectively, under inoculation treatment. These results showed that T. harzianum had the ability to improve soil micro-ecological environment.

Key wordsTrichoderma harzianum  rice  soil microorganism  soil enzymatic activity

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn