摘要:以大豆（Glycine max.）为试验材料，探讨臭氧(O₃)浓度升高〔(80±10) nL·L^-1)对大豆根系内源激素含量及活性氧代谢系统的影响。研究表明：在大豆整个生育期内，O₃胁迫处理下的大豆根系脱落酸（ABA）含量、玉米素核苷（ZR）含量与对照相比显著（P<0.05）升高，生长素（IAA）含量显著（P<0.05）降低，内源激素间平衡改变，IAA/ABA、AR/ABA及(IAA+ZR)/ABA比值显著（P<0.05）降低，超氧阴离子产生速率、MDA含量、POD活性和脯氨酸含量与对照相比显著（P<0.05 ）升高，SOD和CAT活性显著（P<0.05）降低。O₃胁迫在一定程度上可以改变内源激素的含量及激素间的平衡比例，提高大豆的抗氧化能力，但是，长时间的胁迫作用将导致抗氧化系统氧化损伤，从而使膜脂过氧化程度加重，对大豆表现为伤害效应。

关键词:大豆  臭氧胁迫  内源激素  活性氧  抗氧化能力

AbstractThe effects of O₃ concentration elevation 〔(80±10) nL·L^-1〕 on soybean roots endogenous hormones and reactive oxygen metabolism was studied in this paper. Compared with the control，the elevated O₃ concentration induced an increase of abscisic acid (ABA) content and Zeatin riboside (ZR) content (P＜0.05), and a decrease of growth hormone (IAA) content (P＜0.05). The balance of endogenous hormones was changed, and the specific value of IAA/ABA, AR/ABA and (IAA+ZR)/ABA was decreased (P＜0.05). The production rate of superoxide anion, the malondialdehyde (MDA) content POD activity and the proline content were increased (P＜0.05) and the SOD and CAT activity were decreased (P＜0.05). To a certain extent, ozone stress changed the soybean endogenous hormones level and balance and improved its antioxidant ability. However, constant ozone stress could cause the anti-oxidant system damage, exacerbate the membrane lipid peroxidation, and injure the soybean.

Key wordssoybean (Glycine max.)  Ozone stress  plant endogenous hormones  reactive oxygen species  antioxidant ability

文章编号： 0258_7106 (2016) 01_0018_15 中图分类号： P618.41 文献标志码：A

改回日期：2015_07_11

基金项目

**通讯作者耿新霞， 女， 1979年生， 助理研究员， 成矿规律研究方向。 Email： gen gxinxia@cags.ac.cn