引用本文：

高宇，习岗，杨运经，刘锴.极低频高压脉冲电场对萌发绿豆干旱伤害的缓解作用[J].大中文模板3,2014,(6):82-86. [点击复制]
GAO Yu, XI Gang, YANG Yun-jing, LIU Kai.Alleviating effect of extremely low frequency high-voltage pulsed electric field on drought damage of geminating mung beans[J].大中文模板3,2014,(6):82-86. [点击复制]

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极低频高压脉冲电场对萌发绿豆干旱伤害的缓解作用
高宇¹，习岗¹，杨运经²，刘锴¹
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((1.西安理工大学应用物理系，陕西西安 710048; 2. 西北农林科技大学应用物理系，陕西杨凌 712100))

摘要:

采用峰值场强为100 kV·m^-1、脉宽80 ms、脉冲频率为1 Hz的极低频高压脉冲电场（ELF-PEF）处理-0.1 MPa的PEG-6000溶液中萌发的绿豆种子，研究了绿豆在萌发过程中鲜重、自发发光、延迟发光、丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性的变化。结果表明，ELF-PEF对PEG胁迫下萌发的绿豆鲜重、自发发光和延迟发光积分强度促进作用分别达到了28.58%、78.35%和40.6%，说明ELF-PEF可以有效促进绿豆种子萌发过程中的生理代谢，缓解干旱胁迫对细胞的伤害作用。ELF-PEF通过提高PEG胁迫下萌发绿豆中SOD活性，降低MDA含量，缓解干旱胁迫对萌发绿豆的氧化损伤，ELF-PEF对细胞毛细吸水和代谢吸水的促进可能是其缓解干旱伤害的原因。

关键词: 极低频高压脉冲电场萌发绿豆自发发光延迟发光丙二醛超氧化物歧化酶

DOI：

基金项目:国家自然科学基金（50977079，51277151）

Alleviating effect of extremely low frequency high-voltage pulsed electric field on drought damage of geminating mung beans

GAO Yu¹, XI Gang¹, YANG Yun-jing², LIU Kai¹

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Abstract:

Mung beans soaked in -0.1MPa PEG solution were treated by extremely low frequency pulsed electric field （ELF-PEF）with peak electric field strength of 100 kV·m^-1, pulse width of 80 ms, and pulse frequency of 1 Hz. Traits like fresh weight, spontaneous luminescence, delayed luminescence, malondialdehyde (MDA) and superoxide dismutase (SOD) activity during mung beans germination were investigated. The fresh weight, spontaneous luminescence and delayed luminescence integral intensity in germinating mung beans were 28.58%, 78.35% and 40.6%, respectively under PEG stress, indicating that ELF-PEF can accelerate the physiological metabolismand alleviate cells injury under drought stress. Although with increased SOD activity and simultaneously reducing MDA content, the oxidation damage caused by drought stress was alleviated by ELF-PEF in germinating mung beans. The promoting effect of ELF-PEF on capillary water absorption and metabolism water absorption in cells may be the cause of alleviating drought injury.

Key words: extremely low frequency high-voltage pulsed electric field germinating mung beans spontaneous luminescence delayed luminescence malondialdehyde superoxide dismutase