| 引用本文： |
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王新军，谢 静，吴 珍，邓寒霜.秦岭坡旱地不同桔梗栽培品种亲缘关系鉴别[J].大中文模板3,2014,(2):151-156. [点击复制]
- WANG Xin-jun, XIE Jing, WU Zhen, DENG Han-shuang.Identification of the genetic relationship between different cultivated varieties in Qinling Slope dryland[J].大中文模板3,2014,(2):151-156. [点击复制]
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| 摘要: |
| 为对秦岭坡旱地中药材桔梗品种选育及种质资源鉴定提供依据,应用聚丙烯酰胺凝胶电泳(PA
GE)技术研究了不同产地三年生桔梗不同叶位酯酶同工酶(EST)的表达差异，并通过EST-PAGE
图谱迁移率（Rf）、联合系数（S）、酶谱相似度（T）综合分析方法，初步评价了
目前在商洛坡旱地栽培的不同产地桔梗品种的亲缘关系。结果表明：6种不同产地桔梗间的
亲缘关系大小顺序为：甘肃平凉＞山东淄博＞四川广元＞西安蓝田＞内蒙赤峰≈陕西商洛，
内蒙赤峰与陕西商洛桔梗疑为同一品种。 |
| 关键词: 聚丙烯酰胺凝胶电泳 酯酶同工酶 迁移率 联合系数 酶谱相似度 |
| DOI： |
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| 基金项目:陕西省科技厅重点实验室专项项目（008ZY001）；商洛市中药现代化专项基金资助项目（08SKJ001） |
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| Identification of the genetic relationship between different cultivated varieties in Qinling Slope dryland |
| WANG Xin-jun1, XIE Jing2, WU Zhen1, DENG Han-shuang1 |
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| Abstract: |
| In order to provide the basis for variety breeding and germplasm identification of Chinese traditional medicine Platycodon grandiflorum in Qinling slope dryland, platycodon esterase isozymes (EST) expression difference of different leaf position in three different regions is studied
by application of polyacrylamide gel electrophoresis (PAGE) technique, and through the EST-PAGE map mobility (Rf), joint coefficient (S) (T), enzyme spectrum similarity comprehensive analysis method, Put forward the preliminary kinship evaluation of shangluo Platycodon grandiflorum cultivation varieties in the different regions of slope dryland. The results showed that the genetic relationship between different regions size order:Pingliang-Gansu＞
Zibo-Shandong＞Guangyuan-Sichuan＞Lantian-Xian＞Chifeng-Neimenggou≈Shangluo-Shaanxi, there are no difference between Chifeng-Neimenggou and Shangluo-Shaanxi in species of the platycodonis. |
| Key words: polyacrylamide gelelectrop horesis esterase transfer rate joint coefficient enzyme similarity |
