| 引用本文： |
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刘建新，王金成，王瑞娟，贾海燕.旱盐交叉胁迫对燕麦幼苗叶片生理特性的影响[J].大英文模板1,2014,(3):24-28. [点击复制]
- LIU Jian-xin, WANG Jin-cheng, WANG Rui-juan, JIA Hai-yan.Effects of drought and salinity combined stress on activities of physiological characteristics in Avena nuda seedling leaves[J].大英文模板1,2014,(3):24-28. [点击复制]
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| 摘要: |
| 以‘定莜6号’燕麦幼苗为试材，分析了旱盐交叉胁迫下叶片O2·和H2O2产生、膜脂过氧化及抗氧化系统的变化。结果表明，随着干旱和盐胁迫程度的提高，叶片活性氧代谢失调，细胞膜受到严重伤害，表现为SOD、POD和APX活性先升高后降低，AsA含量下降，CAT活性及GSH和丙二醛（MDA）含量升高，O2·和H2O2产生增加。0.3%的土壤含盐量可显著提高轻度（土壤含水量占田间持水量65%）和中度（土壤含水量占田间持水量50%）干旱胁迫下燕麦幼苗叶片SOD、POD和APX的活性，降低O2·产生速率及H2O2和MDA含量；0.6%的土壤含盐量对轻度干旱胁迫燕麦幼苗叶片的O2·产生速率及H
2O2和MDA含量无明显影响，却使中度干旱胁迫下的O2·产生速率及H2O2和MDA含量显著升高；在重度干旱（土壤含水量占田间持水量35%）胁迫下，随着土壤含盐量的增加，燕麦叶片SOD、CAT、POD、APX活性和AsA、GSH含量显著下降，O2·产生速率及H2O2和MDA含量明显增加。表明适量的土壤含盐量可通过提高抗氧化系统的活性缓解
轻、中度干旱胁迫对燕麦幼苗的伤害，而过量的盐分则加重干旱损伤。 |
| 关键词: 燕麦 旱盐交叉胁迫 抗氧化系统 膜脂过氧化 |
| DOI： |
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| 基金项目:甘肃省庆阳市科技支撑计划项目(NK2011-25) |
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| Effects of drought and salinity combined stress on activities of physiological characteristics in Avena nuda seedling leaves |
| LIU Jian-xin, WANG Jin-cheng, WANG Rui-juan, JIA Hai-yan |
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| Abstract: |
| The pot culture experiment was conducted to study the response to drought and salinity combined stress of reactive oxygen species (ROS), including O2· and H2O2, as well as malondialdehyde (MDA) content and antioxidative system in le
aves of naked oat (Avena nuda L. ‘Dingyou No’6) seedlings. The results showed that the cell membrane was seriously damaged due to the disturbed ROS metabolism, activities of cell antioxidant enzymes and antioxidant content. Catalase (CAT) activity and glutathione (GSH) content increased, ascorbic acid (AsA) content decreased. Superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) increased to the peak and then decreased. Furthermore, O2· generation rate and contents of H2O2 and MDA significantly increased in leaves of naked oat seedlings. Compared with the control soil, salinity of 0.3% significantly enhanced the activities of SOD, POD and APX in leaves under the conditions of low drought stress (65% soil field capacity) and medium stress (50% soil field capacity). In addition, soil salinity at 0.6% showed no significant influence on ROS and MDA under mild drought stress. But it significantly increased O2· generation rate and contents of H2O2 and MDA under moderate drought stress. Under severe drought stress (35% soil field capacity), activities of S
OD, CAT, POD and APX, and contents of AsA and GSH in naked oat leaves significantly decreased, while O2· generation rate and contents of H2O2 and MDA increased with degree of salinity stress. These findings suggest that low stress of salinity (0.3%) could partially repair the oxidative damages induced by slight or medium drought stress. |
| Key words: naked oat drought and salinity combined stress antioxidative system lipid peroxidation |
