| 引用本文： |
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王 芳，王丹丹，赵 娟，马 沛，师永沿，王汉宁.钙对低温胁迫下玉米幼苗氧化损伤的保护作用[J].大英文模板1,2014,(1):155-160. [点击复制]
- WANG Fang, WANG Dan-dan, Zhao Juan, MA Pei, SHI Yong-yan, WANG Han-ning.Positive effect of calcium on oxidative damage in maize seedling under chilling stress[J].大英文模板1,2014,(1):155-160. [点击复制]
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| 摘要: |
| 采用室内盆栽试验，利用不同浓度的CaCl2（0、5、10、15、20 mmol·L-1）处理低温胁迫下的玉米幼苗，研究了钙对玉米幼苗抗冷性的作用。结果表明：与对照相比，低温处理明显抑制了玉米幼苗的生长，减缓了可溶性蛋白和可溶性糖含量的积累，抗氧化酶活性下降，脯氨酸含量减少，丙二醛（MDA）含量增加，质膜透性增大。适宜浓度的钙（5～10 mmol·L-1）能减缓低温胁迫下玉米幼苗质膜相对透性和丙二醛（MDA）含量的增加，促进脯氨酸(Pro)、可溶性糖和可溶性蛋白的积累，提高超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性，其中10 mmol·L-1 Ca2+处理的效果最为明显。 |
| 关键词: 玉米幼苗 钙 低温胁迫 氧化损伤 |
| DOI： |
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| 基金项目:甘肃农业大学盛彤笙科技创新基金（GSAU-STS-1229）；973计划前期研究专项（2012CB722902） |
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| Positive effect of calcium on oxidative damage in maize seedling under chilling stress |
| WANG Fang1, WANG Dan-dan1, Zhao Juan1, MA Pei1, SHI Yong-yan2, WANG Han-ning1 |
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| Abstract: |
| The effect of calcium at different concentrations (0, 5, 10, 15 mmol·L-1 and 20 mmol·L-1) on physiological characteristics of maize seedling leaves under chilling stress was studied. The results showed that compared with the CK, the growth of maize seedling were in
hibited and the accumulation of soluble protein and soluble sugar content were maintained, the antioxidant enzymes activities and proline content were reduced, whereas MDA content and membrane permeability were increased under chilling stress. The Ca2+ treatment with the concentration ranging from 5 to 10 mmol·L-1 showed mitigating effect of maintaining membrane permeability and the activities of SOD, POD and CAT, and promoting the accumulation of proline, soluble sugar and soluble protein content in maize seedling. In addition, concentration of 10 mmol·L-1 Ca2+ showed the largest positive influence on seedling growth. |
| Key words: maize seedling calcium chilling stress oxidative damage |
